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After 30 min of blocking with blocking buffer (Dulbeccos PBS containing 0.2% bovine serum albumin, pH 7.4), at RT, the plates were washed 3x with wash buffer (Dulbeccos PBS containing 0.05% Tween 20, pH 7.4)

FINO(2) initiates ferroptosis through GPX4 inactivation and iron oxidation

Antioxidant defense systems can be broadly categorized into four types: preventive antioxidants, radical scavenging antioxidants, repair antioxidant systems, and cellular signalling antioxidant defense systems

There are approximately 2000 species of gut microbiota, and more than 100 species have been identified by phylum classification