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Biochem Biophys Res Commun 266(2):504-511, 1999

Another advantage of this method is that the amide linkages formed by this technique (as well as those created by the NHS method) are more stable than the isourea linkages obtained by CNBr immobilization

Funding This study was financed in part by the Coordenao de Aperfeioamento de Pessoal de Nvel SuperiorCAPES (finance Code 001) and by the Postgraduate Program in Nutrition of the Federal University of Santa Catarina, Brazil

For histological assessment, paraffin tissue sections were stained with hematoxylin and eosin (HE) and Safranin O-Fast Green (SOFG) according to standard laboratory protocols