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Inhibition of the MAPK signaling pathway can activate the LKB1-AMPK signaling pathway, then activate autophagy

High-throughput screening (HTS) using large compound libraries is a powerful strategy for developing new inhibitors, and live cell-based screening has advantages over in vitro assays, including the ability to consider cellular factors such as posttranslational modifications and cofactor concentrations 12,13,14 and differences of membrane permeability of compounds into cells