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The growth medium was replaced every 7 days, and experiments were performed after 14 DIV

In addition, proteasome inhibition, which reduces NF-B activation, has been shown to enhance cellular antioxidant defenses via an Nrf2-dependent transcriptional mechanism, suggesting the indirect effects of NF-B on antioxidant regulation (Karin and Ben-Neriah, 2000

Therefore, the GAS7 oligomer formation on the membrane appeared to be promoted over that in the solution, where the configuration of FFL2 was suggested to be important for the adequate oligomerization of GAS7

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