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We also note that this approach was less practical as it involved more handling steps so using three cycles of freezethaw without buffer replacement would be a preferable approach

Altamura et al

References Laird E, O'Halloran A, Carey D et al (2018) Voluntary Fortification is ineffective to maintain the vitamin B12 and folate status of older Irish adults: Evidence from the Irish Longitudinal Study on Aging (TILDA)