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4.What symptoms would you expect to see in a patient with hereditary mutations in genes coding for proteins of the electron transport chain, such as hereditary defects in cytochrome c oxidase

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After transfer, membranes were incubated with blocking solution (5% milk powder in 1 Tris-buffered saline with Tween 20, pH7.6) for 1 hour and then incubated with primary antibodies (BMAL1 (1:500), CLOCK (1:200), CRY1 (1:1000), CRY2 (1:500), PER 1 (1: 500), PER 2 (1:500), PER 3 (1: 500), NRF2 (1:500), GCLC (1:500), GS (1:1000) or GR (1:500) overnight at 4C