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Supporting this idea, it has been shown that hypoxia might affect mitochondrial cytochromes

ESM1 expression is increased within the TME and, notably, ESM1 reciprocally promotes the progression and maintenance of the TME via the regulation of inflammatory factors, thereby establishing a positive feedback loop between ESM1 and the TME

Protein purification under denaturing conditions: Proteins that are expressed as an inclusion body and have been solubilized with chaotrophic agents such as guanidine-HCl or urea can be purified by modifying the protocol to include the appropriate amount of denaturant (up to 6M guanidine-HCl or up to 8M urea) in the binding, wash and elution buffers

Environmental health perspectives , 2002