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"I can't stand on my leg for a long period," Stewart said

After observing leakiness of the GFP from the ER to the Golgi at the steady state, due to the presence of free biotin coming from DMEM/F-12 and B27-Supplement, we sequestered it by supplying 0.1 mg/ml of NeutrAvidin Protein (Thermo Scientific, #31000) to the WiBi media for 2-3 days before performing live imaging