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Tan CH, Wu Y, Satkunanantham M

After centrifugation, liver protein supernatant was incubated with reaction mixture (0.1 mol/L serine, 5 mmol/L EDTA, and 2.5 mmol/L propargylglycine in Tris buffer (1 mol/L, pH 8.4)), 2.5 mmol/L pyridoxalphosphate and homocysteine reagent for 1 h at 37C

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