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The viability was increased by removing dead cells using the MACS dead cell removal kit according to the manufacturers instructions Blood: Following euthanasia of the mice, a midline laparotomy was performed to expose the abdominal cavity, and the vena cava was carefully isolated and accessed using a 26G needle attached to an Omnifix-F Luer Solo syringe (Braun)

Data availability Bulk RNA sequencing data are available at the European GenomePhenome Archive under code EGAD50000001606

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Therefore, a total of 1749 cases with acquired SNHL and 2018 controls were included