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5.4 Regulation of mitochondrial dysfunction Mitochondria, as subcellular organelles containing DNA, provide energy to cells through oxidative phosphorylation (Antolnez-Fernndez et al., 2024) and are implicated in various pathophysiological processes in VaD

The observed upregulation of Gclc in the liver and pancreatic tissues, following supplementation suggests a potential enhancement of the cellular capacity for glutathione biosynthesis ( Gpx1 ) ( Gpx1 gene expression may reflect a possible increase of the cellular antioxidant defence capacity, as Gpx1 encodes a key glutathione peroxidase enzyme, which is involved in the detoxification of ROS, including hydrogen peroxide and lipid hydroperoxides, thereby potentially contributing to attenuation of oxidative stress ( Ethanol-induced organ damage is characterized by significant inflammation and neutrophil infiltration

Existing preclinical studies in animal models have shown promising results with ferroptosis inhibitors, iron chelation, and microbiota-based therapies such as probiotics, dietary interventions, and fecal microbiota transplantation (FMT)