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Western blot analysis For biochemical analysis of protein activity, another half of the animals in each group were deeply anesthetized with intraperitoneal injection of ketamine (100 mg/kg) and xylazine (10 mg/kg) and sacrificed by cervical dislocation immediately

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However, in MOR, mutation of the two Cys residues does not affect palmitoylation (Chen et al., In the last few months, an important breakthrough has been made with the crystal structures of MOR (Manglik et al., 2012), DOR (Granier et al., 2012) at high resolution

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