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HEK293 cells were seeded in 6 well tissue culture plates and grown to 95% confluency in DMEM containing 10% FBS

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Furthermore, the ability of antibodies generated by priming immunization to trigger the disassembly of recombinant Env protein could generate non-native forms of Env in subsequent immunization steps that are then likely to elicit nnAbs rather than nAbs 30

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