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doi: 10.1016/j.semcancer.2022.03.009 242 ShangYHeYZhangXHeWHuaHYeFet al

For LC-MS analysis, samples were thawed on ice-water bath, then 40 L of whole blood was injected into 450 L cold extraction solvent comprising of mixture of Acetonitrile:Methanol:Milli-Q water in ratio 40:40:20 according to protocol described in Presterud et al 35

( Comparable findings have been reported in models of cerebral ischemia and skeletal muscle injury, where administration of NAC 30 min prior to HBOT suppressed ROS-dependent signaling pathways, including HIF-1 activation, angiogenesis, and tissue regeneration ( In vitro studies further support this concept

Cells were then stained with an anti-mouse PE secondary antibody (BioLegend Cat