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Further studies are required to determine how CaMKII, Rho GTPases, and associated GEFs and GAPs participate in spine formation/elimination under physiological learning conditions in vivo

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I did my own external testing and everything checked out Reggie N

Briefly, 3 g of each protein sample was mixed with 15 L of 50 mM ammonium bicarbonate (pH 7.8) (Thermo Fisher Scientific), followed by protein reduction with 2 L of 100 mM dithiothreitol (Thermo Fisher Scientific) for 30 min and protein alkylation with 5 L of 2-iodoacetamide (Thermo Fisher Scientific) for an additional 30 min at room temperature in the dark