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The machinery is there, but the lights are dim

At the core of the ERMS experiment is the collection of an array of collision-activated decomposition tandem mass spectra (CAD-MS-MS) of a molecular precursor ion, each obtained at a defined value of collision energy, to observe the fall of the intensity of the precursor and the gradual rise of the intensity of the generated fragments

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U1 adaptor inhibit polyA sites and are bifunctional oligonucleotides with a target domain complementary to a site in the target gene's terminal exon and a U1 domain that binds to the U1 smaller nuclear RNA component of the U1 snRNP (Goraczniak, et al., 2008, Nature Biotechnology, 27(3), 257-263, which is expressly incorporated by reference herein, in its entirety)