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(Su, 2016) systematically studied the toxicology and lipid-lowering activity of tobacco seed oil and found that the LD50 of tobacco oil for mice was more than 21,500 mg/kg, and no genotoxicity was found in a Salmonella Typhimurium test, bone marrow cell micronucleus test or chromosome aberration test

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(You can read the methodology here, or view the report in its entirely here