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Regarding diagnostic tools, high-throughput sequencing, predominantly 16S rRNA amplicon sequencing (49), and the integration of multi-omics technologies (including genomics, transcriptomics, proteomics, and metabolomics) (50) have enabled a more comprehensive advancement in exploring the mechanisms by which the gut microbiota and related molecules exert their anti-osteoporosis effects ( Figure 1 )

BCAAs, including leucine, isoleucine, and valine, are essential amino acids involved in energy metabolism and nutrient-sensing pathways [32]

---------- CARNITOR (levocarnitine) is a carrier molecule in the transport of long-chain fatty acids across the inner mitochondrial membrane

Dreher et al., 1997)